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Blotting Quiz Biology

Blotting Quiz Biology

Blotting is technique in which nucleic acids i.e., RNA and DNA or proteins are transferred onto a specific membrane [1,2]. This membrane may be nitrocellulose PVDF or nylon membrane

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Quiz Questions And Answers

Western blotting is the technique for the detection of

specific DNA in a sample
specific RNA in a sample
specific protein in a sample
specific glycolipid in a sample

Which of the following techniques is used in DNA finger printing?

Western blotting
Southern blotting
Northern blotling
Eastern blotting

Labelled antibodies were used to detect

detect the presence of a particular DNA molecule in Southern blotting
detect the presence of a particular RNA atom in Southern blotting
detecting a particular protein molecule in Southern blotting
detecting a particular protein molecule in Western blotting

Which of the following technique doesn't involve electrophoresis for the separation of biomolecules

Dot blotting
Southern blotting
Northern blotting
Western blotting

Which of the following technique is suitable for identifying mRNA molecule in a samle

Western blotting
Southern blotting
Northern blotting

What is pictured above in the DNA replication process?

Replication bubble
Lagging strand
Replication fork

Which strand grows continuously towards the replication fork?

Lagging strand
DNA strand
RNA strand
Leading strand

DNA polymerase III builds new strands from scratch during RNA replication

True
False

Polymerase I enzyme replaces RNA primer on the leading strand with DNA

True
False

The lagging strand is the new strand that grows discontinuously away from the replication fork.

True
False

What is the name of the fragments of the lagging strand pictured above?

Binding proteins
Fragmenting lagging strand
Okazaki segments
Coding strands

What enzyme will solve this problem in the lagging strand? (Pay attention to the red section in the picture).

Ligase
Binding proteins
Helicase
Polymerase I

This vector can be used to construct genomic libraries.

Phage
Cosmid
BAC
All of the above

Generally, cDNA libraries are made using prokaryotic mRNA.

True
False

Usually, special nucleic acid linkers are added to create sticky ends for cloning.

True
False
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