Blotting is technique in which nucleic acids i.e., RNA and DNA or proteins are transferred onto a specific membrane [1,2]. This membrane may be nitrocellulose PVDF or nylon membrane
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Quiz Questions And Answers
Western blotting is the technique for the detection of
Which of the following techniques is used in DNA finger printing?
Labelled antibodies were used to detect
Which of the following technique doesn't involve electrophoresis for the separation of biomolecules
Which of the following technique is suitable for identifying mRNA molecule in a samle
What is pictured above in the DNA replication process?
Which strand grows continuously towards the replication fork?
DNA polymerase III builds new strands from scratch during RNA replication
Polymerase I enzyme replaces RNA primer on the leading strand with DNA
The lagging strand is the new strand that grows discontinuously away from the replication fork.
What is the name of the fragments of the lagging strand pictured above?
What enzyme will solve this problem in the lagging strand? (Pay attention to the red section in the picture).
This vector can be used to construct genomic libraries.
Generally, cDNA libraries are made using prokaryotic mRNA.
Usually, special nucleic acid linkers are added to create sticky ends for cloning.
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