A DNA library is a collection of DNA fragments that have been cloned into vectors so that researchers can identify and isolate the DNA fragments that interest them for further study. There are basically two kinds of libraries: genomic DNA and cDNA libraries
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Quiz Questions And Answers
Gene libraries made from genomic DNA are called genomic libraries and those made from complementary DNA are known as cDNA libraries.
Engineered plasmids are used extensively as vectors for cloning.
This vector can be used to construct genomic libraries.
MRNA can be readily isolated from lysed eukaryotic cells by adding magnetic beads which have _______ covalently attached.
This enzyme is used to dephosphorylate the vector.
Generally, cDNA libraries are made using prokaryotic mRNA.
Usually, special nucleic acid linkers are added to create sticky ends for cloning.
Making cDNA libraries from eukaryotic mRNA is very useful because the cDNAs have no gene sequences.
Since cDNAs are derived from the mRNA, cDNAs represent the transcribed parts of the genome.
In eukaryotes, most mRNAs are polyadenylated and this 3’-tail of about 200 thymine residues provides a useful method for isolating eukaryotic mRNA.
How many types of DNA libraries are possible?
In the following type of libraries pick the odd one out.
Which enzyme is involved in the synthesis of the DNA over an RNA template?
For the construction of DNA library what is the range of genomic DNA that has to be cloned into the vector?
Which of the following enzymes is responsible for the digestion of the RNA strand in the RNA-DNA hybrid?
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