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DNA library Quiz Biology

DNA library Quiz Biology

A DNA library is a collection of DNA fragments that have been cloned into vectors so that researchers can identify and isolate the DNA fragments that interest them for further study. There are basically two kinds of libraries: genomic DNA and cDNA libraries

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Quiz Questions And Answers

Gene libraries made from genomic DNA are called genomic libraries and those made from complementary DNA are known as cDNA libraries.

True
False

Engineered plasmids are used extensively as vectors for cloning.

True
False

This vector can be used to construct genomic libraries.

Plasmid
Phage
Cosmid
All of the above

MRNA can be readily isolated from lysed eukaryotic cells by adding magnetic beads which have _______ covalently attached.

Oligo(dA)
Oligo(dT)
Oligo(dG)
Oligo(dC)

This enzyme is used to dephosphorylate the vector.

Terminal transferase
Alkaline phosphatase
Klenow enzyme
Reverse transcriptase

Generally, cDNA libraries are made using prokaryotic mRNA.

True
False

Usually, special nucleic acid linkers are added to create sticky ends for cloning.

True
False

Making cDNA libraries from eukaryotic mRNA is very useful because the cDNAs have no gene sequences.

True
False

Since cDNAs are derived from the mRNA, cDNAs represent the transcribed parts of the genome.

True
False

In eukaryotes, most mRNAs are polyadenylated and this 3’-tail of about 200 thymine residues provides a useful method for isolating eukaryotic mRNA.

True
False

How many types of DNA libraries are possible?

1
2
3
4

In the following type of libraries pick the odd one out.

A DNA library
B DNA library
cDNA library
Z DNA library

Which enzyme is involved in the synthesis of the DNA over an RNA template?

DNA polymerase
Reverse transcriptase
Klenow fragment
RNA polymerase

For the construction of DNA library what is the range of genomic DNA that has to be cloned into the vector?

Less than 100 bases
100 to 1000 bases
Less than 100 to mega bases
Less than 10 bases

Which of the following enzymes is responsible for the digestion of the RNA strand in the RNA-DNA hybrid?

RNase A
RNase H
DNase
Exonuclease
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